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KMID : 1156220080340050387
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Journal of Environmental Health Sciences
2008 Volume.34 No. 5 p.387 ~ p.394
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Effect of Exposure Concentration and Time of Fuel Additives on the Indigenous Microbial Community in Forests
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Cho Won-Sil
Cho Kyung-Suk
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Abstract
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The toxicity of methyl tert-butyl ether (MTBE), ten-butyl alcohol (TBA) and formaldehyde (FA) on the indigenous microbial community in forest soil was studied. MTBE, TBA and FA with different concentrations were added into microcosms containing forest soil samples. After 10 and 30 days, total viable cell number and dehydrogenase activity in the microcosms were evaluated. Bacterial communities in the microcosms were also analyzed using a denaturing gradient gel electrophoresis (DGGE). Dehydrogenase activity and total viable cell number were decreased according to the increase of MTBE, TBA and FA concentrations (P＜0.05). FA toxicity was the highest, but TBA toxicity was the lowest. The results of principal component analysis using DGGE fingerprints showed that the microbial communities contaminated MTBE, TBA and FA were grouped by exposure time not exposure concentration. Dominant species in the microcosms were as follows: Photobacterium damselae sub sp. and Bacillus sp. KAR28 for MTBE; Mycobacterium sp. and Uncultured Clostridium. sp. for TBA: and Uncultured Paenibacillaceae bacterium and Anxynobacillus, Flavithermus for FA.
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KEYWORD
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Fuel additives, forest soil, methyl tert-butyl ether(MTBE), tert-butyl alcohol(TBA) and formaldehyde(FA), microbial activity and structure
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